This volume collates in one source methodology for in vivo genetic engineering and for genetic analysis in a wide range of bacteria. Not only is Escherichia coli well covered, but so are other emerging bacterial systems.cells by centrifugation (e.g. , 10 min at 6000 rpm in Beckman TJ-6 centrifuge with a fixed-angle rotor). 4. Resuspend the pellet in 5 ml of SMMP with 2 ... with fingers. c. Add 1.5 ml of the PEG solution. Gently mix by rolling the tube manually. d.
Title | : | Bacterial Genetic Systems |
Author | : | Jeffrey H. Miller |
Publisher | : | - 1991 |
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